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ID naloge: 7 Letnik: 1997 Predmet: patologija in histologija
Vpliv spola na eksperimentalno indukcijo tumorjev debelega crevesa
Avtor: Ljubo Breskvar
Mentor: Doc. dr. Anton Cerar
Izhodišca: Karcinom debelega crevesa je pri ljudeh pogostejši pri moških. Raziskave ,
ki obravnavajo pomen vpliva spola na indukcijo kolorektalnega tumorja pri živalih, je malo. Namen naloge je oceniti vpliv spola na indukcijo debelocrevesnega karcinoma z 1,2-dimetilhidazinom (DMH) pri podganah Wistar.
Materiali in metode: Uporabili smo 60 podgan seva Wistar (30 samcev, 30 samic), ki smo jim 1-krat tedensko podkožno vbrizgali 20 mg DMH/kg telesne teže. Injicirali smo 15-krat, po 25 tednih od zacetka poskusa pa smo živali žrtvovali in jih obducirali. Vse makroskopske lezije smo histološko ovrednotili.
Rezultati: Indukcija debelocrevesnih tumorjev je uspela pri 37 % samcev in pri 17 % samic. Našli smo 21 tumorjev debelega crevesa in danke: 15 tumorjev pri samcih in 6 pri samicah. Histološko smo pri samcih našli 11 adenokarcinomov, 2 pecatnocelicna karcinoma in 2 adenoma, pri samicah pa smo našli 4 pecatnocelicne karcinome in 2 adenoma. Našli smo tudi zunaj crevesne tumorje, predvsem tumorje Zymbalovih žlez.
Zakljucki: Podgane seva Wistar v primerjavi z nekaterimi drugimi sevi kažejo manjšo pojavnost kolorektalnih tumorjev po indukciji z DMH. Inducirani tubulni adenokarcinomi in njihova histogeneza pa so primerljivi s cloveškimi KRK. Razlika med spoloma v pojavnosti tumorjev v debelem crevesu in danki se je pokazala kot statisticno mejna (p < 0,08). Statisticno znacilno razliko med spoloma pa smo ugotovili v pojavnosti vseh induciranih tumorjev (p < 0,02). V poskusu smo ugotovili znatno vecjo pojavnost debelocrevesnih tumorjev pri samcih. Poleg tega so bili karcinomi, nastali pri samcih, histološko bolj podobni cloveškim kot tisti, nastali pri samicah. Zato za raziskave tumorjev debelega crevesa bolj priporocamo uporabo Wistar samcev kot samic
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[Abstract / English version]
A role of a gender on the dimethylhydrazine induced colorectal tumors in wistar rats
Author: Ljubo Breskvar
Mentor: Doc. dr. Anton Cerar
BACKGROUND. Human colorectal carcinoma appears more frequently in males. Not much research work has been done about the influence of gender on induction of colorectal carcinoma in animals.
THE PURPOSE of our research was to evaluate the influence on induction of colorectal carcinoma by 1,2-dimethylhydrazine (DMH) in Wistar rats.
METHODS. 60 Wistar rats (30 males, 30 females) were subdued to weekly subcutaneous injections of DMH (20 mg/kg), for 15 weeks. After 25 weeks from the beginning of the experiment the animals were sacrificed and autopsied. All macroscopical lesions were histologicaly evaluated.
RESULTS. Induction of colorectal tumors succeded in 37 % of males and in 17 % of females. 21 tumors of the large bowel were found: 15 of them in males and 6 in females. Histologically, 11 of tumors found in males were adenocarcinomas, 2 were signet-cell carcinomas and 2 were adenomas, whereas 4 signet-cell carcinomas and 2 adenomas were found in females. We also found extracolonic tumors, mainly tumors of the Zymbal glands.
CONCLUSIONS. Wistar strain of rats shows lower incidence of colorectal tumors induced by DMH in comparison with some other strains. The induced tubular adenocarcinomas and their histogenesis are comparable with human colorectal carcinoma.
The gender difference in the incidence of the colorectal tumors was found to be statisticaly marginaly significant (p < 0,08), whereas the difference in the incidence of all induced tumors between genders was statisticaly significant (p < 0,02). Males showed a greater incidence of colorectal tumors and also a greater histological resemblance to human colorectal tumors than females. That is why we recommend Wistar males rather than females for the research work on the colorectal tumors.
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